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Thiol-Based Probe Linker with Antifouling Properties for Aptasensor Development.
- Source :
- Chemosensors; Oct2022, Vol. 10 Issue 10, p435-N.PAG, 18p
- Publication Year :
- 2022
-
Abstract
- Surfaces with antifouling properties are critical for optimizing biosensors to improve the selectivity and specificity of analyte detection in complex biological samples. This work describes the four-step synthesis of 3-dithiothreitol propanoic acid (DTT<subscript>COOH</subscript>), a new antifouling thiol linker that (a) significantly reduces fouling of raw human serum samples and (b) binds amino receptors via its terminal carboxylic acid group. DTT<subscript>COOH</subscript> was successfully functionalized on quartz crystal microbalance (QCM) discs and used to anchor penicillin-binding aptamers. Relative to bare and coated (11-mercaptoundecanoic acid (MUA) and 1-undecanethiol (UDT)) QCM crystals, DTT<subscript>COOH</subscript>'s antifouling improved by approximately 75–86%. Following aptamer/ethanolamine extension, the modified DTT<subscript>COOH</subscript> layer reduced serum fouling by approximately 95–97% compared to bare and coated (MUA, UDT) crystals. QCM with dissipation (QCM-D) monitoring, contact goniometry, and cyclic voltammetry techniques were used to compare the DTT<subscript>COOH</subscript> surfaces with quartz crystals functionalized with hydrophobic and hydrophilic molecules. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 22279040
- Volume :
- 10
- Issue :
- 10
- Database :
- Complementary Index
- Journal :
- Chemosensors
- Publication Type :
- Academic Journal
- Accession number :
- 159904025
- Full Text :
- https://doi.org/10.3390/chemosensors10100435