High‐throughput sequencing revealed low‐efficacy genome editing using Cas9 RNPs electroporation and single‐celled microinjection provided an alternative to deliver CRISPR reagents into Euglena gracilis.

GLO:F16/01nov22:pbi13915-fig-0001.jpg PHOTO (COLOR): 1 CRISPR genome editing to target the EgGSL2 gene by electroporation and the crtP1 gene using microinjection to deliver Cas9-RNPs into E. gracilis. Keywords: Euglena gracilis; CRISPR; Gene editing; Electroporation; Microinjection EN Euglena gracilis CRISPR Gene editing Electroporation Microinjection 2048 2050 3 11/01/22 20221101 NES 221101 The genus I Euglena i contains more than 1000 species of single-celled flagellated microorganisms with both plant and animal characteristics. High-throughput sequencing revealed low-efficacy genome editing using Cas9 RNPs electroporation and single-celled microinjection provided an alternative to deliver CRISPR reagents into Euglena gracilis. [Extracted from the article]