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A type I DnaJ homolog, DjA1, regulates androgen receptor signaling and spermatogenesis.
- Source :
- EMBO Journal; 2/9/2005, Vol. 24 Issue 3, p611-622, 12p
- Publication Year :
- 2005
-
Abstract
- Two type I DnaJ homologs DjA1 (DNAJA1; dj2, HSDJ/hdj-2, rdj1) and DjA2 (DNAJA2; dj3, rdj2) work similarly as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro. To study the in vivo role of DjA1, we generated DjA1-mutant mice. Surprisingly, loss of DjA1 in mice led to severe defects in spermatogenesis that involve aberrant androgen signaling. Transplantation experiments with green fluorescent protein-labeled spermatogonia into DjA1<superscript>-/-</superscript> mice revealed a primary defect of Sertoli cells in maintaining spermiogenesis at steps 8 and 9. In Sertoli cells of DjA1<superscript>-/-</superscript> mice, the androgen receptor markedly accumulated with enhanced transcription of several androgen-responsive genes, including Pem and testin. Disruption of Sertoli-germ cell adherens junctions was also evident in DjA1<superscript>-/-</superscript> mice. Experiments with DjA1<superscript>-/-</superscript> fibroblasts and primary Sertoli cells indicated aberrant androgen receptor signaling. These results revealed a critical role of DjA1 in spermiogenesis and suggest that DjA1 and DjA2 are not functionally equivalent in vivo. [ABSTRACT FROM AUTHOR]
- Subjects :
- ANDROGENS
ANDROSTANE
SEX hormones
SPERMATOGENESIS
TESTIS
GAMETOGENESIS
Subjects
Details
- Language :
- English
- ISSN :
- 02614189
- Volume :
- 24
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- EMBO Journal
- Publication Type :
- Academic Journal
- Accession number :
- 16010135
- Full Text :
- https://doi.org/10.1038/sj.emboj.7600549