EVALUATION OF THE INHIBITORY ACTIVITY OF A NANOHYBRID CHLORHEXIDINE ANTISEPTIC AGAINST S. AUREUS AND A. BAUMANNII ISOLATED FROM WOUNDS.

The purpose the research was to create a nano hybrid from Chlorhexidine antiseptic and evaluation of its inhibitory effect against Staphylococcus aureus and Acinetobacter baumannii from different wounds. A nanohybrid antiseptic was prepared using Chlorhexidine (Chx) and Magnesium oxide (MgO). A novel nano antimicrobial has been identified. FT-IR spectroscopy revealed the appearance of new groups in the Chx-MgO spectrum, which indicates the formation of a new compound. The X-Ray Diffraction (XRD) spectrum revealed the appearance of new diffraction planes in the nano hybrid antibiotic spectrum in compare with spectrum of MgO. The three-dimensional image of the above compound in atomic force microscope confirmed the formation of new antiseptic with nano dimensions. The nanohybrid antiseptic's antibacterial activity was tested against 11 S. aureus isolates and seven A. baumannii isolates. The outcomes revealed that the highest level of inhibition was achieved against 4 isolates having an inhibitory zone on average of 33.5 mm with Chx-MgO and 2 isolates with average inhibition zone of 35.5 mm at 1024 µg/ml concentration against S. aureus. As well as 27.5 mm using Chx-MgO as average inhibition zone at 1024 µg/ml concentration and 2 isolates with average inhibition zone of 23.5 mm with Chx-free in the same concentration against A. baumannii. In conclusion, the antiseptic effectiveness of a nanohybrid Chlorhexidine antiseptic against isolated S. aureus and A.baumannii. [ABSTRACT FROM AUTHOR]