EFFECTS OF DIFFERENT DOSES OF GLUTATHIONE AND GLUTATHIONE COMBINED WITH CISPLATIN ON VALUES OF P53 PROTEIN AND MICRONUCLEUS IN ALBINO MICE.

Reduced Glutathione (GSH) was found in all mammalian tissues, as antioxidant is plays an important role in number of biochemical processes that includes repair of oxidativestress damage, defense against free radicals, apoptosis, signal transduction and gene expression. Cisplatin (CDDP) as common chemotherapy used against many forms of human cancers. Unfortunately, like many chemotherapy drugs the CDDP have adverse effects on cancers patients such as cytotoxicity on normal tissue and decreases levels of glutathione. The current studywas verified the effect of GSH by using different dosages (10, 20, 30 and 40 mg/kg) on Swiss albino mice, then chosen the best dose of GSH to use with one acute dose (2.5mg/kg) of CDDP, through measuring two parameters: Micronucleus rate MN as Cytotoxic test and Tumor Suppressor Protein p53 levels as a Genotoxic test. Results of combination group showed significant decrease in number of micronuclei (7.48%) compared to CDDP group (9.64%) and significant increase in levels of p53(164.63pg/ml) compared to CDDP group (47.26 pg/ml). Although, results when compared to control group (2.60%) it still significant increases in formation of MN rate, also significant increases in p53 values when compared to control group (72.27pg/ml). This means that GSH may be has ability to improve the cytotoxicity of CDDP and reduces its toxicity on normal cells of cancers patients. [ABSTRACT FROM AUTHOR]