The Effect of Anthocyanins from Dioscorea alata L. on Antioxidant Properties of Perinatal Hainan Black Goats and Its Possible Mechanism in the Mammary Gland.

Simple Summary: As they are the primary meat goats of Hainan, how to safely and stably transport Hainan black goats through the perinatal period has become one of the major issues. Likewise, anthocyanin-rich Dioscorea alata L. is grown to some extent in Hainan but is wasted in great quantities. The aim of this paper is to explore the effect of Dioscorea alata L. anthocyanins (DAC) on the antioxidant capacity of perinatal Hainan black goats, as well as the possible mechanism on the effect of prior protection, thereby enhancing the reproductive ability of goats and developing characteristic forage resources. Our results indicated feeding a diet in which Dioscorea alata L. powder replaces 30% of corn can increase the antioxidant capacity of perinatal female goats and newborn kids. Furthermore, under H₂O₂ stress, the use of DAC to treat goat mammary epithelial cells (GMEC) may serve an effective pre-protective function. This will provide data to support the protection of perinatal black goat females and the development of specific food resources. (1) Background: The mammary glands of the perinatal goats are susceptible to reactive oxygen species (ROS) leading to oxidative injury. Although Dioscorea alata L. is rich in anthocyanins with high safety and excellent free-radical-scavenging ability, the effect and mechanism of Dioscorea alata L. anthocyanins (DAC) on the antioxidant capacity of the black Hainan goat has been the subject of few studies to date; (2) Methods: For this reason, feeding experiments were performed by feeding experimental diets, and the pre-protective capacity of DAC on goat mammary epithelial cells was explored on the basis of the established model of H₂O₂ injury; (3) Results: As well as altering rumen fermentation parameters in perinatal female goats, dietary challenge also improves antioxidant capacity in their blood and milk. thereby enhancing children's antioxidant capacity and increasing their resistance to oxidative stress. However, we also found that DAC pretreatment was capable of activating both Nrf2 and MAPK/JNK pathways, which results in enhanced antioxidase activity and elimination of ROS; (4) Conclusions: Together, these findings suggest that DAC may have a pre-protective role on perinatal Hainan black goats through the regulation of Nrf2 and MAPK/JNK pathways in GMEC. [ABSTRACT FROM AUTHOR]