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H2-induced transient upregulation of phospholipids with suppression of energy metabolism.
- Source :
- Medical Gas Research; Sep2023, Vol. 13 Issue 3, p133-141, 9p
- Publication Year :
- 2023
-
Abstract
- Molecular hydrogen (H<subscript>2</subscript>) is an antioxidant and anti-inflammatory agent; however, the molecular mechanisms underlying its biological effects are largely unknown. Similar to other gaseous molecules such as inhalation anesthetics, H<subscript>2</subscript> is more soluble in lipids than in water. A recent study demonstrated that H<subscript>2</subscript> reduces radical polymerization-induced cellular damage by suppressing fatty acid peroxidation and membrane permeability. Thus, we sought to examine the effects of short exposure to H<subscript>2</subscript> on lipid composition and associated physiological changes in SH-SY5Y neuroblastoma cells. We analyzed cells by liquid chromatography-high-resolution mass spectrometry to define changes in lipid components. Lipid class analysis of cells exposed to H<subscript>2</subscript> for 1 hour revealed transient increases in glycerophospholipids including phosphatidylethanolamine, phosphatidylinositol, and cardiolipin. Metabolomic analysis also showed that H<subscript>2</subscript> exposure for 1 hour transiently suppressed overall energy metabolism accompanied by a decrease in glutathione. We further observed alterations to endosomal morphology by staining with specific antibodies. Endosomal transport of cholera toxin B to recycling endosomes localized around the Golgi body was delayed in H<subscript>2</subscript>-exposed cells. We speculate that H<subscript>2</subscript>-induced modification of lipid composition depresses energy production and endosomal transport concomitant with enhancement of oxidative stress, which transiently stimulates stress response pathways to protect cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- ENERGY metabolism
PHOSPHOLIPIDS
GOLGI apparatus
CHOLERA toxin
INHALATION anesthetics
Subjects
Details
- Language :
- English
- ISSN :
- 20459912
- Volume :
- 13
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Medical Gas Research
- Publication Type :
- Academic Journal
- Accession number :
- 161016510
- Full Text :
- https://doi.org/10.4103/2045-9912.344973