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ROSETTE FORMATION BY MOUSE LYMPHOCYTES I. DEMONSTRATION BY INDIRECT IMMUNOFLUORESCENCE OF T CELLS BINDING SHEEP ERYTHROCYTES.

Authors :
Gyöngyössy, Maria I. C.
Playfair, J. H. L.
Source :
Clinical & Experimental Immunology; Oct1974, Vol. 18 Issue 2, p169-176, 8p
Publication Year :
1974

Abstract

A method is described for indirect immunofluorescent staining of mouse T cells forming rosettes, involving fixation of rosettes by gluturaldehyde and staining with a rabbit anti-mouse brain serum absorbed so as to be specific for T cells. Using this technique, it was shown that about 44%, of spleen cells forming rosettes with sheep erythrocytes both in non-immunized and immunized mice, are T cells. The number of T rosette-forming cells increased about ten-fold after immunization with sheep erythrocytes. but only when serum antibody was detectable. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00099104
Volume :
18
Issue :
2
Database :
Complementary Index
Journal :
Clinical & Experimental Immunology
Publication Type :
Academic Journal
Accession number :
16118783