Monoclonal Anti-Azobenzenearsonate Antibodies Expressing the Cross-reactive Idiotype: Immunochemical Studies Show that All Idiotypic Determinants Reside on a Single Molecule.

Cell lines that secreted antibodies to the hapten azobenzencarsonate IAHA) were established by hybridization of immune A.J spleen cells to the non-secreting myeloma. NS-1. Solid-phase radioimmunoassays (Risk) were developed for rapid screening of hybridoma supernatants to detect antibodies to ABA and to detect antibodies bearing the ABA cross-reactive idiotype (CR1). Hybrid clones secreting both CR1 and CR1 anti-ABA antibodies were obtained. The supernatant from one clone (7-1-3 p strongly inhibited binding of iodinated anti-idiotype serum in at competitive Risk. This clone expressing the CR1 produced immunoglobulin of the IgG2a sub- class. Solid-phase absorption of anti-idiotype serum followed by competitive radioimrnunoassay analyses revealed that all the idiotypye determinants recognized by anti-idiotype serum reside on this manoclonal antibody. [ABSTRACT FROM AUTHOR]