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Comparison of DNA methylation profiles of human embryos cultured in either uninterrupted or interrupted incubators.
- Source :
- Journal of Assisted Reproduction & Genetics; Jan2023, Vol. 40 Issue 1, p113-123, 11p
- Publication Year :
- 2023
-
Abstract
- Purpose: We aimed to compare the DNA methylation profiles of human embryos cultured in uninterrupted or interrupted incubators. Methods: This study included 9 women, ≤ 30 years old (range: 20–30 years), without a history of genetic diseases or smoking, undergoing ICSI treatment, and each woman donated one oocyte. Embryos were randomly assigned to culture in either time-lapse imaging or standard incubators after ICSI. We compared the DNA methylation profiles of human eight-cell embryos cultured in uninterrupted condition using time-lapse imaging (TLI) incubator (EmbryoScope) to those cultured in interrupted culture model using standard incubators (SI, G185 K-System). Nine single-cell whole-genome bisulfite sequencing (WGBS) datasets were analyzed, including four SI-cultured embryos and five TLI-cultured embryos at the eight-cell stage. Results: A total of 581,140,020 and 732,348,182 clean reads were generated from the TLI and SI groups, respectively. TLI-cultured embryos had similar genome-wide methylation patterns to SI-cultured embryos. There were no significant differences in the methylation and transcription levels of transposable elements and imprinted control regions. Although a total of 198 differentially methylated genes (DMGs) were identified, only five DMGs had significantly different transcription levels between the two groups. Conclusions: This is the first study to compare the DNA methylation profiles of embryos cultured in TLI and SI and will provide a foundation for evaluating the safety of TLI application in assisted reproductive technologies. However, further study with a larger cohort of samples was needed for the data validation. [ABSTRACT FROM AUTHOR]
- Subjects :
- HUMAN embryos
DNA methylation
METHYLATION
INCUBATORS
REPRODUCTIVE technology
DNA
OVUM
Subjects
Details
- Language :
- English
- ISSN :
- 10580468
- Volume :
- 40
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Assisted Reproduction & Genetics
- Publication Type :
- Academic Journal
- Accession number :
- 161300836
- Full Text :
- https://doi.org/10.1007/s10815-022-02669-9