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Expression of a heterologous expansin in transgenic tomato plants.
- Source :
- Planta: An International Journal of Plant Biology; Sep2000, Vol. 211 Issue 4, p583-586, 4p
- Publication Year :
- 2000
-
Abstract
- Expansins are cell wall proteins thought to play an important role in growth and other events involving cell wall modifications. Whereas the expression patterns of many isoforms have been characterised, the nature of their activity is still poorly understood. Large amounts of active expansins are necessary to undertake biochemical studies and identify their substrates. We report here the successful expression of a recombinant expansin [CsExp1, isolated from cucumber (Cucumis sativus L.) hypocotyls] in transgenic tomato (Lycopersicon esculentum Mill.) plants, under the control of a constitutive promoter. In some transformants, CsExp1 transcript and protein accumulated to high levels, and expansin activity extractable from the cell walls was increased up to about 20-fold the activity measured in wild-type plants. These results confirm the identity of the CsExp1 coding sequence, and will enable large quantities of active expansin to be obtained for further studies. [ABSTRACT FROM AUTHOR]
- Subjects :
- TOMATOES
TECHNICAL specifications
NATURE
CUCUMBERS
BIOMOLECULES
PLANT cell walls
Subjects
Details
- Language :
- English
- ISSN :
- 00320935
- Volume :
- 211
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Planta: An International Journal of Plant Biology
- Publication Type :
- Academic Journal
- Accession number :
- 16131970
- Full Text :
- https://doi.org/10.1007/s004250000319