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Evaluation of RT-LAMP Assay for Rapid Detection of SARS-CoV-2.
- Source :
- Laboratory Medicine; Jan2023, Vol. 54 Issue 1, p56-64, 9p
- Publication Year :
- 2023
-
Abstract
- Objective To evaluate the accuracy of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in community or primary-care settings. Method We systematically searched the Web of Science, Embase, PubMed, and Cochrane Library databases. We conducted quality evaluation using ReviewManager software (version 5.0). We then used MetaDisc software (version 1.4) and Stata software (version 12.0) to build forest plots, along with a Deeks funnel plot and a bivariate boxplot for analysis. Result Overall, the sensitivity, specificity, and diagnostic odds ratio were 0.79, 0.97, and 328.18, respectively. The sensitivity for the subgroup with RNA extraction appeared to be higher, at 0.88 (0.86–0.90), compared to the subgroup without RNA extraction, at 0.50 (0.45–0.55), with no significant difference in specificity. Conclusion RT-LAMP assay exhibited high specificity regarding current SARS-CoV-2 infection. However, its overall sensitivity was relatively moderate. Extracting RNA was found to be beneficial in improving sensitivity. [ABSTRACT FROM AUTHOR]
- Subjects :
- ONLINE information services
MEDICAL databases
COVID-19
MOLECULAR diagnosis
META-analysis
MEDICAL information storage & retrieval systems
CONFIDENCE intervals
SYSTEMATIC reviews
COMMUNITY health services
PRIMARY health care
DESCRIPTIVE statistics
COVID-19 testing
SENSITIVITY & specificity (Statistics)
MEDLINE
DATA analysis software
ODDS ratio
LOGISTIC regression analysis
NUCLEIC acid amplification techniques
EARLY diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 00075027
- Volume :
- 54
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Laboratory Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 161404444
- Full Text :
- https://doi.org/10.1093/labmed/lmac030