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Microfluidic cell engineering on high-density microelectrode arrays for assessing structure-function relationships in living neuronal networks.

Authors :
Yuya Sato
Hideaki Yamamoto
Hideyuki Kato
Takashi Tanii
Shigeo Sato
Ayumi Hirano-Iwata
Source :
Frontiers in Neuroscience; 1/9/2023, Vol. 16, p01-11, 11p
Publication Year :
2023

Abstract

Neuronal networks in dissociated culture combined with cell engineering technology offer a pivotal platform to constructively explore the relationship between structure and function in living neuronal networks. Here, we fabricated defined neuronal networks possessing a modular architecture on high-density microelectrode arrays (HD-MEAs), a state-of-the-art electrophysiological tool for recording neural activity with high spatial and temporal resolutions. We first established a surface coating protocol using a cell-permissive hydrogel to stably attach a polydimethylsiloxane microfluidic film on the HD-MEA. We then recorded the spontaneous neural activity of the engineered neuronal network, which revealed an important portrait of the engineered neuronal network--modular architecture enhances functional complexity by reducing the excessive neural correlation between spatially segregated modules. The results of this study highlight the impact of HDMEA recordings combined with cell engineering technologies as a novel tool in neuroscience to constructively assess the structure-function relationships in neuronal networks. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
16624548
Volume :
16
Database :
Complementary Index
Journal :
Frontiers in Neuroscience
Publication Type :
Academic Journal
Accession number :
161461098
Full Text :
https://doi.org/10.3389/fnins.2022.943310