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INHIBITION OF IN VITRO LYMPHOCYTE BLASTOGENESIS BY INHIBITOR PRODUCED BY CULTURED HUMAN LYMPHOBLASTS.
- Source :
- Clinical & Experimental Immunology; Jul1974, Vol. 17 Issue 3, p463-473, 11p
- Publication Year :
- 1974
-
Abstract
- Normal human lymphoblasioid cell lines, growing in continuous suspension culture, produce inhibitors of in vitro lymphocyte blastogenesis. The inhibitor reduces human lymphocyte blastogenic responses to phytohaemagglutinin, slreptolysin-O and the mixed lymphocyte culture 90-99%, is non-cytotoxic and can inhibit both newly initiated and on-going responses. The inhibitor is heat-stable at 80°C but labile at 100°C, non-dialysable and degraded by pronase but not DNase or RNase. It is species- and tissue-specific and does not inhibit the proliferation of mouse lymphocytes, human melanoma cells or human bone marrow in vitro colony-forming cells. Inhibitor was produced only under very specific conditions of crowding. Thus, maximal inhibitor production occurred at 5 × 10<superscript>6</superscript> lymphocytes per cm" culture surface area while only 0-5%, of the maximal amount was produced at 10<superscript>6</superscript> or 5 &tiems; 10<superscript>7</superscript> lymphoblasts per cm10<superscript>2</superscript>. This data is relevant to the nature of feedback control of immunological reactions and may guide the development of new classes of immunosuppressants. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00099104
- Volume :
- 17
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Clinical & Experimental Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 16173636