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Characterization of cytokine gene expression in CD4+ and CD8+ T cells after activation with phorbol myristate acetate and phytohaemagglutinin.
- Source :
- Clinical & Experimental Immunology; Oct1992, Vol. 90 Issue 1, p147-153, 7p
- Publication Year :
- 1992
-
Abstract
- Cytokines are important mediators involved in the development of effector cells and in the regulation of immune responses. The gene expression of these mediators in T cell subset has yet to be fully elucidated. Using sensitive reverse transcription-polymerase chain reaction (RT-PCR), the kinetics of cytokine gene expression in human CD4<superscript>+</superscript> and CD8<superscript>+</superscript> T cells were examined. CD4<superscript>+</superscript> T cells were more readily activated by phorbol myristate acetate (PMA) and phytohaemagglutinin (PHA) than CD8<superscript>+</superscript> T cells in terms of the IL-2 receptor (IL-2R) mRNA expression. Quantitative differences in cytokine gene expression between CD4<superscript>+</superscript> and CD8<superscript>+</superscript> T cells were confirmed and higher levels of cytokine mRNAs were induced in CD4<superscript>+</superscript> than in CD8<superscript>+</superscript> T cells. Early induction of IL-2 mRNA was observed in both T cell subsets. The demonstration of different kinetics of cytokine gene expression illustrates one of the examples of the complexity of immunoregulation. The differential response of cytokine gene expression in different T cell subsets should be taken into consideration when clinical studies in cytokine production by peripheral blood mononuclear cells are interpreted. [ABSTRACT FROM AUTHOR]
- Subjects :
- CYTOKINES
CELLS
MESSENGER RNA
T cells
CELLULAR immunity
IMMUNOREGULATION
Subjects
Details
- Language :
- English
- ISSN :
- 00099104
- Volume :
- 90
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Clinical & Experimental Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 16177303