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Mass cytometry analysis identifies T cell immune signature of aplastic anemia and predicts the response to cyclosporine.
- Source :
- Annals of Hematology; Mar2023, Vol. 102 Issue 3, p529-539, 11p
- Publication Year :
- 2023
-
Abstract
- Aplastic anemia (AA) is an auto-activated T cell–mediated bone marrow failure. Cyclosporine is often used to treat non-severe AA, which demonstrates a more heterogeneous condition than severe AA. The response rate to cyclosporine is only around 50% in non-severe AA. To better predict response to cyclosporine and pinpoint who is the appropriate candidate for cyclosporine, we performed phenotypic and functional T cell immune signature at single cell level by mass cytometry from 30 patients with non-severe AA. Unexpectedly, non-significant differences of T cell subsets were observed between AA and healthy control or cyclosporine-responder and non-responders. Interestingly, when screening the expression of co-inhibitory molecules, T cell trafficking mediators, and cytokines, we found an increase of cytotoxic T lymphocyte antigen 4 (CTLA-4) on T cells in response to cyclosporine and a lower level of CTLA-4 on CD8<superscript>+</superscript> T cells was correlated to hematologic response. Moreover, a decreased expression of sphingosine-1-phosphate receptor 1 (S1P<subscript>1</subscript>) on naive T cells and a lower level of interleukin-9 (IL-9) on T helpers also predicted a better response to cyclosporine, respectively. Therefore, the T cell immune signature, especially in CTAL-4, S1P<subscript>1</subscript>, and IL-9, has a predictive value for response to cyclosporine. Collectively, our study implies that immune signature analysis of T cell by mass cytometry is a useful tool to make a strategic decision on cyclosporine treatment of AA. [ABSTRACT FROM AUTHOR]
- Subjects :
- T cells
APLASTIC anemia
CYTOTOXIC T cells
CYCLOSPORINE
CYTOMETRY
T cell receptors
Subjects
Details
- Language :
- English
- ISSN :
- 09395555
- Volume :
- 102
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Annals of Hematology
- Publication Type :
- Academic Journal
- Accession number :
- 162181014
- Full Text :
- https://doi.org/10.1007/s00277-023-05097-6