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Conversion of Escherichia coli into Mixotrophic CO 2 Assimilation with Malate and Hydrogen Based on Recombinant Expression of 2-Oxoglutarate:Ferredoxin Oxidoreductase Using Adaptive Laboratory Evolution.
- Source :
- Microorganisms; Feb2023, Vol. 11 Issue 2, p253, 12p
- Publication Year :
- 2023
-
Abstract
- We report the mixotrophic growth of Escherichia coli based on recombinant 2-oxoglutarate:ferredoxin oxidoreductase (OGOR) to assimilate CO<subscript>2</subscript> using malate as an auxiliary carbon source and hydrogen as an energy source. We employ a long-term (~184 days) two-stage adaptive evolution to convert heterotrophic E. coli into mixotrophic E. coli. In the first stage of evolution with serine, diauxic growth emerges as a prominent feature. At the end of the second stage of evolution with malate, the strain exhibits mixotrophy with CO<subscript>2</subscript> as an essential substrate for growth. We expect this work will open new possibilities in the utilization of OGOR for microbial CO<subscript>2</subscript> assimilation and future hydrogen-based electro-microbial conversion. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20762607
- Volume :
- 11
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Microorganisms
- Publication Type :
- Academic Journal
- Accession number :
- 162379148
- Full Text :
- https://doi.org/10.3390/microorganisms11020253