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IN VITRO STUDIES OF LYMPHOCYTES FROM PATIENTS WITH PLASMA CELL MYELOMA.
- Source :
- Clinical & Experimental Immunology; Nov1973, Vol. 15 Issue 3, p321-329, 9p
- Publication Year :
- 1973
-
Abstract
- Highly purified blood lymphocytes from treated and untreated patients with plasmacell myeloma were tested for the presence of *EAC lymphocytes carrying the C3 receptor surface marker using a rosette-technique and for thymus-derived (T) lymphocytes as judged by spontaneous rosette formation with sheep red blood cells. The treated patients were tested 5 weeks after a standardized 4-day treatment with melphalan and prednisolone. Untreated patients had 40-65% EAC-lymphocytes in peripheral blood while the EAC-cell level in treated patients was < 20%. A non-myeloma control group had 20-40%. The percentage of EAC-lymphocytes was correlated with different in vitro functional tests: lymphocyte-mediated lysis of target cells (Chang cell line or chicken red blood cells) treated with rabbit antibody correlated with the percentage of EAC-lymphocytes. DNA synthesis induced in lymphocytes by pokeweed mitogen (PWM) showed a biphasic dose response curve which may indicate activition of two populations of lymphocytes. A correlation was found between EAC-lymphocytes and DNA synthesis induced by a low concentration of PWM (0.1 μg/ml). The results support observations by other investigators which suggest that antibody-induced cytotoxicity requires cells in the non-T-lymphocyte population and the assumption that PWM is in part a mitogen for human bone marrow-derived lymphocytes. [ABSTRACT FROM AUTHOR]
- Subjects :
- LEUCOCYTES
MYELOMA proteins
BLOOD cells
CELL culture
ERYTHROCYTES
IMMUNE system
Subjects
Details
- Language :
- English
- ISSN :
- 00099104
- Volume :
- 15
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Clinical & Experimental Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 16243638