Study of some properties of the receptor for IgM on human lymphocytes.

Some properties of the receptor for IgM on human lymphocytes have been investigated. It was shown that the interaction of native IgM with the receptor present on T and B lymphocytes is not critical for its detection in the EA_M-rosette assay. In fact, high values of EA_M-RFC could be found on cell suspensions cultured overnight in either IgM-free or IgM-containing media. In addition, the inhibition of EA_M-rosettes by human monoclonal IgM at 37°C was not as effective as at 4°C. Rabbit IgM showed a significantly greater ability to inhibit the binding of antigen-IgM antibody complexes than human IgM. The receptor for IgM was easily removed by handling procedures, the incubation of lymphocytes at 4°C and treatment of the cells with low concentrations of trypsin or pronase. After the enzymatic treatment, a rapid resynthesis occurred, which restored the number of EA_M-rosettes formed by T cells and significantly increased the number formed by B cells. The interaction between the receptor and antigen-IgM antibody complexes stopped the spontaneous shedding of the receptor at 4°C. When the incubation of the cells with immune complexes was performed at 37°C, a .significantly different behaviour between T cells equipped with receptor for IgM and those possessing receptor for IgG was found. After the binding of EA_G to the receptor for IgG, a process of rapid dissociation of rosettes occurred, whereas the incubation with EA_M did not induce an irreversible loss of the receptor for IgM. [ABSTRACT FROM AUTHOR]