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Study of some properties of the receptor for IgM on human lymphocytes.
- Source :
- Clinical & Experimental Immunology; Jun1979, Vol. 36 Issue 3, p502-510, 9p
- Publication Year :
- 1979
-
Abstract
- Some properties of the receptor for IgM on human lymphocytes have been investigated. It was shown that the interaction of native IgM with the receptor present on T and B lymphocytes is not critical for its detection in the EA<subscript>M</subscript>-rosette assay. In fact, high values of EA<subscript>M</subscript>-RFC could be found on cell suspensions cultured overnight in either IgM-free or IgM-containing media. In addition, the inhibition of EA<subscript>M</subscript>-rosettes by human monoclonal IgM at 37°C was not as effective as at 4°C. Rabbit IgM showed a significantly greater ability to inhibit the binding of antigen-IgM antibody complexes than human IgM. The receptor for IgM was easily removed by handling procedures, the incubation of lymphocytes at 4°C and treatment of the cells with low concentrations of trypsin or pronase. After the enzymatic treatment, a rapid resynthesis occurred, which restored the number of EA<subscript>M</subscript>-rosettes formed by T cells and significantly increased the number formed by B cells. The interaction between the receptor and antigen-IgM antibody complexes stopped the spontaneous shedding of the receptor at 4°C. When the incubation of the cells with immune complexes was performed at 37°C, a .significantly different behaviour between T cells equipped with receptor for IgM and those possessing receptor for IgG was found. After the binding of EA<subscript>G</subscript> to the receptor for IgG, a process of rapid dissociation of rosettes occurred, whereas the incubation with EA<subscript>M</subscript> did not induce an irreversible loss of the receptor for IgM. [ABSTRACT FROM AUTHOR]
- Subjects :
- LYMPHOCYTES
LEUCOCYTES
CELLS
T cells
THERAPEUTICS
IMMUNE complexes
Subjects
Details
- Language :
- English
- ISSN :
- 00099104
- Volume :
- 36
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Clinical & Experimental Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 16246633