The Rapid Detection of Bongkrekic Acid in Foods Using Colloidal Gold Immunochromatographic Assay.

In this study, a specific monoclonal antibody against bongkrekic acid (BA) was produced. The antibody was conjugated to colloidal gold as a tracer to develop a colloidal gold immunochromatography assay (GICA). The effect of working conditions, including pH value of labeling, mass concentrations of antibody and antigen, ionic strength, surfactant as well as sample pretreatment on GICA performance were studied. Under the optimal condition, the developed GICA showed a quantitative limit of detection of 1.2 µg/kg, a detection range of 1.8-7.2 µg/kg, and a qualitative detection limit of 16.0 µg/kg, respectively. The proposed assay has shown good specificity, and exhibited no cross-reaction with other toxins commonly found in food. Recovery test was done by using tremella, agaric, and rice flour samples and ranged from 80.2% to 101.2% with the relative standard deviation (RSD) less than 6.4%. Besides, the results had good agreement with that of liquid chromatography-tandem mass spectrometry (LC-MS/MS). In conclusion, the proposed assay can meet the demand for mass screening of bongkrekic acid in food in an accurate, sensitive, convenient, rapid, and low-cost manner. [ABSTRACT FROM AUTHOR]