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Diagnostic performance of metagenomic next-generation sequencing in non-tuberculous mycobacterial pulmonary disease when applied to clinical practice.
- Source :
- Infection; Apr2023, Vol. 51 Issue 2, p397-405, 9p, 1 Color Photograph, 2 Diagrams, 1 Chart, 1 Graph
- Publication Year :
- 2023
-
Abstract
- Objective: To compare non-tuberculous mycobacterial pulmonary disease (NTMPD) diagnosis by metagenomic next-generation sequencing (mNGS) with Bactec mycobacterial growth indicator tube (MGIT) 960. Methods: A total of 422 patients with suspected NTMPD in Shanghai Pulmonary Hospital between January 2020 and May 2021 were retrospectively analyzed; 194 were diagnosed with NTMPD. The diagnostic performance of mNGS and MGIT 960 for NTMPD was assessed. Receiver operating characteristic (ROC) curves and areas under curve (AUCs) were compared. Results: The sensitivity of mNGS in NTMPD diagnosis was 81.4% and higher than that of MGIT 960 (53.6%). The specificity of mNGS in NTMPD diagnosis was 97.8%, similar to that of MGIT 960 (100%). The sensitivity of combined mNGS and MGIT 960 in NTMPD diagnosis was 91.8%. The sensitivity of mNGS for bronchoalveolar lavage fluid (BALF), pulmonary puncture tissue fluid, and sputum was 84.8%, 80.6%, and 77.5%, respectively; all were higher than that of MGIT 960 (P < 0.05). The AUC of mNGS and MGIT 960 was 0.897 and 0.768, respectively. The AUC of mNGS were BALF (0.916), pulmonary puncture tissue fluid (0.903), and sputum (0.870). Conclusion: The sensitivity of mNGS was superior to that of Bactec MGIT 960; the specificity in NTMPD diagnosis was similar. mNGS shows effective performance in NTMPD diagnosis. [ABSTRACT FROM AUTHOR]
- Subjects :
- MYCOBACTERIAL disease diagnosis
LUNG disease diagnosis
BODY fluid analysis
SEQUENCE analysis
BRONCHOALVEOLAR lavage
SPUTUM
RETROSPECTIVE studies
PHARMACOKINETICS
COMPARATIVE studies
GENOMICS
DESCRIPTIVE statistics
RESEARCH funding
RECEIVER operating characteristic curves
SENSITIVITY & specificity (Statistics)
OLIGONUCLEOTIDE arrays
MICROBIAL sensitivity tests
Subjects
Details
- Language :
- English
- ISSN :
- 03008126
- Volume :
- 51
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Infection
- Publication Type :
- Academic Journal
- Accession number :
- 162700295
- Full Text :
- https://doi.org/10.1007/s15010-022-01890-z