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SangeR: the high-throughput Sanger sequencing analysis pipeline.
- Source :
- Bioinformatics Advances; 2022, Vol. 2 Issue 1, p1-2, 2p
- Publication Year :
- 2022
-
Abstract
- Summary In the era of next generation sequencing and beyond, the Sanger technique is still widely used for variant verification of inconclusive or ambiguous high-throughput sequencing results or as a low-cost molecular genetical analysis tool for single targets in many fields of study. Many analysis steps need time-consuming manual intervention. Therefore, we present here a pipeline-capable high-throughput solution with an optional Shiny web interface, that provides a binary mutation decision of hotspots together with plotted chromatograms including annotations via flat files. Availability and implementation SangeR is freely available at https://github.com/Neuropathology-Giessen/SangeR and https://hub.docker.com/repository/docker/kaischmid/sange%5fr Contact Kai.Schmid@patho.med.uni-giessen.de or Daniel.Amsel@patho.med.uni-giessen.de Supplementary information Supplementary data are available at Bioinformatics online. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- Volume :
- 2
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Bioinformatics Advances
- Publication Type :
- Academic Journal
- Accession number :
- 162786259
- Full Text :
- https://doi.org/10.1093/bioadv/vbac009