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Comparison of N2O-reducing abilities and genome features of two nosZ-containing denitrifying bacteria, Pseudomonas veronii DM15 and Pseudomonas frederiksbergensis DM22.
- Source :
- Journal of Applied Microbiology; Jan2023, Vol. 134 Issue 1, p1-9, 9p
- Publication Year :
- 2023
-
Abstract
- Aim The study systematically compared the N<subscript>2</subscript>O-reducing functional performances and the genomic features of two N<subscript>2</subscript>O-reducing isolates, aimed to screen out effective N<subscript>2</subscript>O-reducing bacteria with strong environmental adaption, and explore the possible regulation. Methods and Results Two N<subscript>2</subscript>O reducers, namely, Pseudomonas veronii DM15 (DM15) and Pseudomonas frederiksbergensis DM22 (DM22), isolated from paddy soil were selected. Their N<subscript>2</subscript>O-reducing abilities, and nosZ gene transcript abundance were determined under different temperatures (20°C, 30°C, 40°C) and oxygen concentrations (0%, 10%, 21%), and the whole genomes were sequenced by Illumina sequencing. The results showed that both DM15 and DM22 exhibited the strongest N<subscript>2</subscript>O reducing activity at 30°C and under anaerobic conditions. In comparison, DM15 generally exhibited significantly higher N<subscript>2</subscript>O reducing abilities and nosZ gene expression than DM22 under all tested conditions. In addition, DM15 possessed obviously higher expression potentials (codon adaptation index (CAI) value) of nos genes than DM22, and the nos cluster of the former contained a transcriptional regulator gene of dnr , while the latter did not. Conclusions The results indicate that DM15 showed obviously stronger N<subscript>2</subscript>O-reducing abilities than DM22 under various conditions, which might be closely associated with its dnr transcriptional regulator, and thus promoting the higher transcriptional activities of nos genes. Although anaerobic conditions were the optimal conditions for N<subscript>2</subscript>O reduction in both strains, DM15 still reduced a certain amount of N<subscript>2</subscript>O even under aerobic conditions. [ABSTRACT FROM AUTHOR]
- Subjects :
- DENITRIFYING bacteria
PSEUDOMONAS
GENE expression
REGULATOR genes
GENOMES
Subjects
Details
- Language :
- English
- ISSN :
- 13645072
- Volume :
- 134
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Applied Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 162823906
- Full Text :
- https://doi.org/10.1093/jambio/lxac011