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miR394 enhances WUSCHEL‐induced somatic embryogenesis in Arabidopsis thaliana.

Authors :
Lu, Lu
Holt, Anna
Chen, Xinying
Liu, Yang
Knauer, Steffen
Tucker, Elise J.
Sarkar, Ananda Kumar
Hao, Zhaodong
Roodbarkelari, Farshad
Shi, Jisen
Chen, Jinhui
Laux, Thomas
Source :
New Phytologist; May2023, Vol. 238 Issue 3, p1059-1072, 14p
Publication Year :
2023

Abstract

Summary: Many plant species can give rise to embryos from somatic cells after a simple hormone treatment, illustrating the remarkable developmental plasticity of differentiated plant cells. However, many species are recalcitrant to somatic embryo formation for unknown reasons, which poses a significant challenge to agriculture, where somatic embryogenesis is an important tool to propagate desired genotypes. The micro‐RNA394 (miR394) promotes shoot meristem maintenance in Arabidopsis thaliana, but the underlying mechanisms have remained elusive.We analyzed whether miR394 affects indirect somatic embryogenesis and determined the transcriptome of embryogenic callus upon miR394‐enhanced somatic embryogenesis.We show that ectopic miR394 expression enhances somatic embryogenesis in the recalcitrant Ler accession when co‐expressed with the transcription factor WUSCHEL (WUS) and that miR394 acts in this process through silencing the target LEAF CURLING RESPONSIVENESS (LCR). Furthermore, we show that higher endogenous miR394 levels are required for the elevated embryogenic potential of the Columbia accession compared with Ler, providing a mechanistic explanation for this natural variation.Our transcriptional analysis provides a framework for miR394 function in regulating pluripotency by expanding WUS‐mediated direct transcriptional repression. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
0028646X
Volume :
238
Issue :
3
Database :
Complementary Index
Journal :
New Phytologist
Publication Type :
Academic Journal
Accession number :
162824483
Full Text :
https://doi.org/10.1111/nph.18801