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Ethanol Extract of Glycyrrhiza uralensis Fisch: Antidiarrheal Activity in Mice and Contraction Effect in Isolated Rabbit Jejunum.
- Source :
- Chinese Journal of Integrative Medicine; Apr2023, Vol. 29 Issue 4, p325-332, 8p
- Publication Year :
- 2023
-
Abstract
- Objective: To evaluate the antidiarrheal effect of ethanol extract of Glycyrrhiza uralensis Fisch root (GFR) in vivo and jejunal contraction in vitro. Methods: In vivo, 50 mice were divided into negative control, positive control (verapamil), low-, medium- and high-dose GFR (250, 500, 1,000 mg/kg) groups by a random number table, 10 mice in each group. The antidiarrheal activity was evaluated in castor oil-induced diarrhea mice model by evacuation index (EI). In vitro, the effects of GFR (0.01, 0.03, 0.1, 0.3, 1, 3, and 10 g/L) on the spontaneous contraction of isolated smooth muscle of rabbit jejunum and contraction of pretreated by Acetylcholine (ACh, 10 µmol/L) and KCl (60 mmol/L) were observed for 200 s. In addition, CaCl<subscript>2</subscript> was accumulated to further study its mechanism after pretreating jejunal smooth muscle with GFR (1 and 3 g/L) or verapamil (0.03 and 0.1 µmol/L) in a Ca<superscript>2+</superscript>-free-high-K<superscript>+</superscript> solution containing ethylene diamine tetraacetic acid (EDTA). Results: GFR (500 and 1,000 mg/kg) significantly reduced EI in castor oil-induced diarrhea model mice (P<0.01). Meanwhile, GFR (0.01, 0.03, 0.1, 0.3, 1, 3, and 10 g/L) inhibited the spontaneous contraction of rabbit jejunum (P<0.05 or P<0.01). Contraction of jejunums samples pretreated by ACh and KCl with 50% effective concentration (EC<subscript>50</subscript>) values was 1.05 (0.71–1.24), 0.34 (0.29–0.41) and 0.15 (0.11–0.20) g/L, respectively. In addition, GFR moved the concentration-effect curve of CaCl<subscript>2</subscript> down to the right, showing a similar effect to verapamil. Conclusions: GFR can effectively against diarrhea and inhibit intestinal contraction, and these antidiarrheal effects may be based on blocking L-type Ca<superscript>2+</superscript> channels and muscarinic receptors. [ABSTRACT FROM AUTHOR]
- Subjects :
- GLYCYRRHIZA
IN vitro studies
BIOLOGICAL models
GASTROINTESTINAL motility
DIARRHEA
JEJUNUM
SMOOTH muscle
MUSCLE contraction
IN vivo studies
ANIMAL experimentation
ANTIDIARRHEALS
RABBITS
PLANT roots
TREATMENT effectiveness
DESCRIPTIVE statistics
RESEARCH funding
PLANT extracts
CHINESE medicine
MICE
PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 16720415
- Volume :
- 29
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Chinese Journal of Integrative Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 162869062
- Full Text :
- https://doi.org/10.1007/s11655-022-3536-5