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Cross-linked enzyme aggregates immobilization: preparation, characterization, and applications.

Authors :
Chen, Ning
Chang, Baogen
Shi, Nian
Yan, Wenxing
Lu, Fuping
Liu, Fufeng
Source :
Critical Reviews in Biotechnology; May2023, Vol. 43 Issue 3, p369-383, 15p
Publication Year :
2023

Abstract

Enzymes are commonly used as biocatalysts for various biological and chemical processes. However, some major drawbacks of free enzymes (e.g. poor reusability and instability) significantly restrict their industrial practices. How to overcome these weaknesses remain considerable challenges. Enzyme immobilization is one of the most effective ways to improve the reusability and stability of enzymes. Cross-linked enzyme aggregates (CLEAs) has been known as a novel and versatile carrier-free immobilization method. CLEAs is attractive due to its simplicity and robustness, without purification. It generally shows: high catalytic specificity and selectivity, good operational and storage stabilities, and good reusability. Moreover, co-immobilization of different kinds of enzymes can be acquired. These CLEAs advantages provide opportunities for further industrial applications. Herein, the preparation parameters of CLEAs were first summarized. Next, characterization of structural and catalytic properties, stability and reusability are also proposed. Finally, some important applications of this technique in: environmental protection, industrial chemistry, food industry, and pharmaceutical synthesis and delivery are introduced. Potential challenges and future research directions, such as improving cross-linking efficiency and internal mass transfer efficiency, are also presented. This implies that CLEAs provide an efficient and feasible technique to improve the properties of enzymes for use in the industry. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
07388551
Volume :
43
Issue :
3
Database :
Complementary Index
Journal :
Critical Reviews in Biotechnology
Publication Type :
Academic Journal
Accession number :
162967587
Full Text :
https://doi.org/10.1080/07388551.2022.2038073