Synthesis, characterization and DNA binding properties of 3,6-bis[(alkylamino)ureido]acridines.

New four 3,6-bis[(alkylamino)ureido]acridines were synthesized and thoroughly characterized using NMR spectra. In vitro DNA binding studies of acridines carried out employing absorption, fluorescence and circular dichroism measurements revealed their binding into DNA via intercalation. The UV–Vis absorbance data were analysed to obtain the binding constant (K_b). K_b values were found in the range of 4.46 − 6.73 × 10⁴ M⁻¹. The DNA viscosity measurements were also performed to confirm the binding mode of the investigated acridines. [ABSTRACT FROM AUTHOR]