Back to Search
Start Over
The Cystic Fibrosis Transmembrane Conductance Regulator Gene (CFTR) Is under Post-Transcriptional Control of microRNAs: Analysis of the Effects of agomiRNAs Mimicking miR-145-5p, miR-101-3p, and miR-335-5p.
- Source :
- Non-Coding RNA; Apr2023, Vol. 9 Issue 2, p29, 11p
- Publication Year :
- 2023
-
Abstract
- (1) Background: MicroRNAs are involved in the expression of the gene encoding the chloride channel CFTR (Cystic Fibrosis Transmembrane Conductance Regulator); the objective of this short report is to study the effects of the treatment of bronchial epithelial Calu-3 cells with molecules mimicking the activity of pre-miR-145-5p, pre-miR-335-5p, and pre-miR-101-3p, and to discuss possible translational applications of these molecules in pre-clinical studies focusing on the development of protocols of possible interest in therapy; (2) Methods: CFTR mRNA was quantified by Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR). The production of the CFTR protein was assessed by Western blotting; (3) Results: The treatment of Calu-3 cells with agomiR-145-5p caused the highest inhibition of CFTR mRNA accumulation and CFTR production; (4) Conclusions: The treatment of target cells with the agomiR pre-miR-145-5p should be considered when CFTR gene expression should be inhibited in pathological conditions, such as polycystic kidney disease (PKD), some types of cancer, cholera, and SARS-CoV-2 infection. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 2311553X
- Volume :
- 9
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Non-Coding RNA
- Publication Type :
- Academic Journal
- Accession number :
- 163455366
- Full Text :
- https://doi.org/10.3390/ncrna9020029