Back to Search
Start Over
miR-181c, a potential mediator for acute kidney injury in a burn rat model with following sepsis.
- Source :
- European Journal of Trauma & Emergency Surgery; Apr2023, Vol. 49 Issue 2, p1035-1045, 11p, 1 Chart, 5 Graphs
- Publication Year :
- 2023
-
Abstract
- Background: The miRNA profile is changed after burn or sepsis and is involved in regulating inflammatory reactions. However, the function and molecular mechanism of miRNAs in regulating burn sepsis-induced acute kidney injury (AKI) are still unclear. Methods: In this study, animal and cell sepsis models were established after burned rats were injected with lipopolysaccharide (LPS) or NRK-52E cells treated with LPS, respectively. Cytokine expression, inflammatory cell infiltration, serum creatinine (Scr) and kidney injury molecule-1 (KIM-1) levels were analysed after the indicated treatments. Results: Burn sepsis increased the expression of inflammatory factors (TNF-α and IL-1β) and chemokines (MIP-1α, MIP-2 and MCP-1). Moreover, burn sepsis promoted macrophage and neutrophil infiltration into the kidney and upregulated the levels of Scr and KIM-1 in the kidney and urine. Ectopic expression of miR-181c significantly reduced LPS-induced TLR4 protein expression, suppressed KIM-1 mRNA levels and subsequently inhibited the activation of inflammatory genes (TNF-α and IL-1β) and chemokine genes (MIP-1α, MIP-2 and MCP-1). Conclusions: Our results demonstrated that miR-181c could suppress TLR4 expression, reduce inflammatory factor and chemokine secretion, mitigate inflammatory cell infiltration into the kidney and downregulate KIM-1 expression, which might ultimately attenuate burn sepsis-induced AKI. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 18639933
- Volume :
- 49
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- European Journal of Trauma & Emergency Surgery
- Publication Type :
- Academic Journal
- Accession number :
- 163721473
- Full Text :
- https://doi.org/10.1007/s00068-022-02124-5