Clinical applicability of miR517a detection in liquid biopsies of ETMR patients.

To develop a robust and easily applicable miRNA detection method, we established a qRT-PCR method to detect miR517a and validated its expression across 11 ETMR tumor tissues as well as controls (Fig. MiR517a was significantly higher in blood of ETMR patients as compared to samples of non-tumor bearing controls and of medulloblastoma patients (MB) (Fig. To develop liquid biopsy tools for ETMR, we performed a comprehensive analysis of cell-free DNA as well as miRNA in cerebrospinal fluid and blood of ETMR patients (Fig. CNV of miR517a detected in tumor DNA and cfDNA from CSF for case#5 and case#9. d Swimmer plot of 5 ETMR patients, including all dates of surgery, liquid biopsies, and MRIs. e Illustration of experimental workflow (created with BioRender). [Extracted from the article]