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Taxonogenomics of Culturomica massiliensis gen. nov., sp. nov., and Emergencia timonensis gen. nov., sp. nov. new bacteria isolated from human stool microbiota.
- Source :
- Scientific Reports; 5/25/2023, Vol. 13 Issue 1, p1-11, 11p
- Publication Year :
- 2023
-
Abstract
- Two new bacterial strains, Marseille-P2698<superscript>T</superscript> (CSUR P2698 = DSM 103,121) and Marseille-P2260<superscript>T</superscript> (CSUR P2260 = DSM 101,844 = SN18), were isolated from human stools by the culturomic method. We used the taxonogenomic approach to fully describe these two new bacterial strains. The Marseille-P2698<superscript>T</superscript> strain was a Gram-negative, motile, non-spore-forming, rod-shaped bacterium. The Marseille-P2260<superscript>T</superscript> strain was a Gram-positive, motile, spore-forming rod-shaped bacterium. Major fatty acids found in Marseille-P2698<superscript>T</superscript> were C<subscript>15:0 iso</subscript> (63%), C<subscript>15:0 anteiso</subscript> (11%), and C<subscript>17:0 3-OH iso</subscript> (8%). Those found in Marseille-P2260<superscript>T</superscript> strain were C<subscript>16:00</subscript> (39%), C<subscript>18:1n9</subscript> (16%) and C<subscript>18:1n7</subscript> (14%). Strains Marseille-P2698<superscript>T</superscript> and Marseille-P2260<superscript>T</superscript> had 16S rRNA gene sequence similarities of 91.50% with Odoribacter laneus<superscript>T</superscript>, and of 90.98% and 95.07% with Odoribacter splanchnicus<superscript>T</superscript> and Eubacterium sulci<superscript>T</superscript>, respectively. The exhibited digital DNA-DNA Hybridization values lower than 20.7%, and Orthologous Average Nucleotide Identity values lower than 73% compared to their closest related bacterial species O. splanchnicus<superscript>T</superscript> and E. sulci<superscript>T</superscript> respectively. Phenotypic, biochemical, phylogenetic, and genomic results obtained by comparative analyses provided sufficient evidence that both of the two studied strains Marseille-P2698<superscript>T</superscript> and Marseille-P2260<superscript>T</superscript> are two new bacterial species and new bacterial genera for which the names Culturomica massiliensis gen. nov., sp. nov., and Emergencia timonensis gen. nov., sp. nov. were proposed, respectively. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 13
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Scientific Reports
- Publication Type :
- Academic Journal
- Accession number :
- 163913887
- Full Text :
- https://doi.org/10.1038/s41598-023-35443-7