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Construction of a rapid electrochemical biosensor consisting of a nanozyme/aptamer conjugate for waterborne microcystin detection.
- Source :
- Analyst; 6/7/2023, Vol. 148 Issue 11, p2536-2543, 8p
- Publication Year :
- 2023
-
Abstract
- Microcystin-LR (MC-LR) is a hepatotoxin generated by the excessive proliferation of cyanobacteria, which is a threat to humans and wildlife. Therefore, rapid detection of MC-LR is an important challenge. This study describes a rapid electrochemical biosensor comprising nanozymes and aptamers. Alternating current electrothermal flow (ACEF) significantly reduced the MC-LR detection period to 10 min. We also used MnO<subscript>2</subscript>/MC-LR aptamer conjugates to improve the sensitivity to MC-LR detection. Here, MnO<subscript>2</subscript> amplified the electrochemical signal and the aptamer showed high selectivity for MC-LR. Under the optimal conditions, the limit of detection (LOD) and selectivity in freshwater were detected using cyclic voltammetry and differential pulse voltammetry. As a result, an LOD of 3.36 pg mL<superscript>−1</superscript> was observed in the linear concentration range of 10 pg mL<superscript>−1</superscript> to 1 μg mL<superscript>−1</superscript>. This study quickly and sensitively detected MC-LR in a situation where it causes serious damage worldwide. In addition, the ACEF technology introduction is the first example of MC-LR detection, suggesting a wide range of possibilities for MC-LR biosensors. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032654
- Volume :
- 148
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- Analyst
- Publication Type :
- Academic Journal
- Accession number :
- 163983882
- Full Text :
- https://doi.org/10.1039/d3an00391d