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iAstrocytes do not restrain T cell proliferation in vitro.
- Source :
- BMC Neuroscience; 6/7/2023, Vol. 24 Issue 1, p1-9, 9p
- Publication Year :
- 2023
-
Abstract
- The cross-talk between T cells and astrocytes occurring under physiological and, even more, neuroinflammatory conditions may profoundly impact the generation of adaptive immune responses in the nervous tissue. In this study, we used a standardized in vitro co-culture assay to investigate the immunomodulatory properties of astrocytes differing for age, sex, and species. Mouse neonatal astrocytes enhanced T cell vitality but suppressed T lymphocyte proliferation in response to mitogenic stimuli or myelin antigens, regardless of the Th1, Th2 or Th17 T cell phenotype. Studies comparing glia cells from adult and neonatal animals showed that adult astrocytes were more efficient in inhibiting T lymphocyte activation than neonatal astrocytes, regardless of their sex. Differently from primary cultures, mouse and human astrocytes derived from reprogrammed fibroblasts did not interfere with T cell proliferation. Overall, we describe a standardized astrocyte-T cell interaction in vitro assay and demonstrate that primary astrocytes and iAstrocytes may differ in modulating T cell function. [ABSTRACT FROM AUTHOR]
- Subjects :
- T cells
CELL proliferation
T helper cells
CELL physiology
NERVE tissue
Subjects
Details
- Language :
- English
- ISSN :
- 14712202
- Volume :
- 24
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- BMC Neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 164150696
- Full Text :
- https://doi.org/10.1186/s12868-023-00806-3