Studies from University of Georgia in the Area of West Nile Fever Reported (Storage Time and Temperature of Filter Paper Strips Affect Anti-west Nile Virus Antibody Detection In 2 Galliform Species).

We collected sera and FPSs from 23 wild turkeys (Meleagris gallopavo) and 20 northern bobwhites (Colinus virginianus) experimentally infected with West Nile virus (WNV) to compare FPS storage methods with WNV plaque reduction neutralization test (PRNT) sensitivity." FPS eluates and sera were co-titrated to determine endpoint antibody titers, which were compared between FPS sera eluted immediately and sera, and among FPS eluates that underwent different storage conditions. Antibody titers were similar to 4-fold less in FPS sera eluted immediately versus sera, and dropped more frequently below PRNT detection threshold in northern bobwhites, which had similar to 10-fold lower serum antibody titers than wild turkeys. [Extracted from the article]