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In vivo normobaric oxygen exposure depresses spleen cell in vitro Con A response. Effects of 2-mercaptoethanol and peritoneal cells.
- Source :
- Clinical & Experimental Immunology; Nov1984, Vol. 58 Issue 2, p428-435, 8p
- Publication Year :
- 1984
-
Abstract
- Normobaric O<subscript>2</subscript> exposure decreased spleen cell (SC) response to T cell mitogen Con A. ³H-TdR incorporation of SC from O<subscript>2</subscript> exposed mice (O<subscript>2</subscript>SC) compared to those of control mice (Air SC) decreased significantly after 72 and 87 h O<subscript>2</subscript> exposure. The dose response kinetics to Con A were identical in O<subscript>2</subscript>SC or Air SC. Increasing SC number did not restore the response to Con A and the depressed hyperoxic effect was not related to suppressor cells in the spleen of O<subscript>2</subscript> exposed mice. Response of O<subscript>2</subscript>SC to Con A was restored by the thiol compound 2-niercaptoethanol (2-ME). and the degree of restoration by 2-ME. was inversely proportional to the depressed response. Addition of intact peritoneal cells (PC) induced restoration within the same range as 2-ME. Restoration of the mitogenic response by 2-ME involved antioxidant properties and suggested that macrophages were functionally injured by O<subscript>2</subscript> exposure. In cases where mitogen response was highly depressed, restoration was only partial; in these conditions in vivo O<subscript>2</subscript> injury probably involved both macrophages and splenic T cells. The mechanisms of O<subscript>2</subscript> toxicity have been discussed in terms of free radical generation under hyperoxic conditions. [ABSTRACT FROM AUTHOR]
- Subjects :
- SPLEEN
HEMATOPOIETIC system
LYMPHOID tissue
T cells
CELLS
LYMPHOCYTES
Subjects
Details
- Language :
- English
- ISSN :
- 00099104
- Volume :
- 58
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Clinical & Experimental Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 16437522