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A novel hACE2 knock-in mouse model recapitulates pulmonary and intestinal SARS-CoV-2 infection.
- Source :
- Frontiers in Microbiology; 2023, p01-12, 12p
- Publication Year :
- 2023
-
Abstract
- Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission is responsible for the coronavirus disease 2019 (COVID-19) pandemic. SARS-CoV-2 uses the angiotensin-converting enzyme 2 (ACE2) receptor to enter the host, and the gastrointestinal tract is a potential infection site as this receptor is expressed on it. Multiple studies have indicated that an increasing number of COVID-19 patients presented with gastrointestinal symptoms that are highly associated with disease severity. Moreover, emerging evidence has demonstrated that alterations in the gut immune microenvironment induced by intestinal SARS-CoV-2 infection can regulate respiratory symptoms. Therefore, targeting the intestines may be a candidate therapeutic strategy in patients with COVID-19; however, no mouse model can serve as an appropriate infection model for the development of fatal pneumonia while mimicking intestinal infection. In this study, a novel human ACE2 knock-in (KI) mouse model (or hACE2-KI) was systemically compared with the popular K18-hACE2 mice; it showed differences in the distribution of lung and intestinal infections and pathophysiological characteristics. These newly generated hACE2-KI mice were susceptible to intranasal infection with SARS-CoV-2, and not only developed mild to severe lung injury, but also acquired intestinal infection. Consequently, this model can be a useful tool for studying intestinal SARS-CoV-2 infection and developing effective therapeutic strategies. [ABSTRACT FROM AUTHOR]
- Subjects :
- SARS-CoV-2
LUNGS
INTESTINAL infections
COVID-19
LABORATORY mice
ANIMAL disease models
Subjects
Details
- Language :
- English
- ISSN :
- 1664302X
- Database :
- Complementary Index
- Journal :
- Frontiers in Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 164443740
- Full Text :
- https://doi.org/10.3389/fmicb.2023.1175188