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Ultrasensitive Silicon Nanowire Biosensor with Modulated Threshold Voltages and Ultra-Small Diameter for Early Kidney Failure Biomarker Cystatin C.

Authors :
Hu, Jiawei
Li, Yinglu
Zhang, Xufang
Wang, Yanrong
Zhang, Jing
Yan, Jiang
Li, Junjie
Zhang, Zhaohao
Yin, Huaxiang
Wei, Qianhui
Jiang, Qifeng
Wei, Shuhua
Zhang, Qingzhu
Source :
Biosensors (2079-6374); Jun2023, Vol. 13 Issue 6, p645, 14p
Publication Year :
2023

Abstract

Acute kidney injury (AKI) is a frequently occurring severe disease with high mortality. Cystatin C (Cys-C), as a biomarker of early kidney failure, can be used to detect and prevent acute renal injury. In this paper, a biosensor based on a silicon nanowire field-effect transistor (SiNW FET) was studied for the quantitative detection of Cys-C. Based on the spacer image transfer (SIT) processes and channel doping optimization for higher sensitivity, a wafer-scale, highly controllable SiNW FET was designed and fabricated with a 13.5 nm SiNW. In order to improve the specificity, Cys-C antibodies were modified on the oxide layer of the SiNW surface by oxygen plasma treatment and silanization. Furthermore, a polydimethylsiloxane (PDMS) microchannel was involved in improving the effectiveness and stability of detection. The experimental results show that the SiNW FET sensors realize the lower limit of detection (LOD) of 0.25 ag/mL and have a good linear correlation in the range of Cys-C concentration from 1 ag/mL to 10 pg/mL, exhibiting its great potential in the future real-time application. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20796374
Volume :
13
Issue :
6
Database :
Complementary Index
Journal :
Biosensors (2079-6374)
Publication Type :
Academic Journal
Accession number :
164581331
Full Text :
https://doi.org/10.3390/bios13060645