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Enhancement of Human B-Cell Proliferation by a Monoclonal Antibody to CD43.
- Source :
- Scandinavian Journal of Immunology; Mar1989, Vol. 29 Issue 3, p363-370, 8p
- Publication Year :
- 1989
-
Abstract
- Monoclonal antibodies (MoAb) to human leucocyte sialoglycoprotein, CD43, have been shown to deliver mitogenic signals to human T cells or to enhance T-cell proliferation induced by concanavalin A, anti-CD3 antibodies or phorbolester. In this paper, we studied the effects of anti-CD43 MoAb B1B6 on the activation of human B cells. Anti-CD43 MoAb B1B6 was not mitogenic by itself for human B cells. However, when added together with TPA, was not mitogenic by itself for human B cells, containing 5-10% and about 35% CD43<superscript>+</superscript>, respectively, responded with three- to fivefold higher proliferation compared to that obtained with TPA alone. A peak in the proliferative response was reached on day 3. Optimal proliferation was obtained when the antibody was present from the start of culturing. Addition of MoAb B1B6 together with a calcium ionophore, ionomycin, did not induced B-cell proliferation. Neither did mAb B1B6 sustain the growth of B cells that were already in the cell cycle, i.e. precultured with phorbol ester (PDB) and ionomycin for 3 days. The results are similar to those obtained with antibodies to CD22 and CD23 and show that early progression signals are delivered to resting B cells through CD43 in the presence of primary activation of protein kinase C. [ABSTRACT FROM AUTHOR]
- Subjects :
- LYMPHOCYTES
CELLS
B cells
CELL division
CELL proliferation
IMMUNOGLOBULINS
Subjects
Details
- Language :
- English
- ISSN :
- 03009475
- Volume :
- 29
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Scandinavian Journal of Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 16458631
- Full Text :
- https://doi.org/10.1111/j.1365-3083.1989.tb01135.x