MoS 2 @Au as Label for Sensitive Sandwich-Type Immunoassay of Neuron-Specific Enolase.

Neuron-specific enolase (NSE) has gained extensive attention as a reliable target for detecting small cell carcinoma of lungs. In this paper, an electrochemical immunoassay method based on molybdenum disulfide (MoS₂) is proposed to detect NSE sensitively. By an in-situ growth method, MoS₂ and Au nanoclusters (Au NCs) were composited to form a MoS₂@Au nanozyme, and then the secondary antibodies were modified. Primary antibodies were immobilized on amino-reduced graphene oxides to capture NSE. The flower-like MoS₂ nanozyme provided abundant sites to load Au NCs and catalyze the decomposition of H₂O₂, which were beneficial to amplify an amperometric response as well as build up sensitivity. Under optimum conditions, the detection range of this strategy was 0.1 pg·mL⁻¹–10 ng·mL⁻¹ and the limit of detection was 0.05 pg·mL⁻¹. This sensing strategy achieved the prospect of sensitively detecting NSE. Moreover, the prepared electrochemical immunosensor provides a theoretical basis and technical support for the detection of other disease markers. [ABSTRACT FROM AUTHOR]