"Virus Purification Method Using Apatite Column" in Patent Application Approval Process (USPTO 20230212530).

The purification method according to claim 1, wherein the first elution step comprises the pH gradient elution, the first adsorbent comprising fluoroapatite, and the second elution step comprises the salt concentration gradient elution, the second adsorbent comprising hydroxyapatite. Keywords: Apatites; Bioengineering; Biological Products; Biomedical Engineering; Biomedicine; Biotechnology; Business; Drugs and Therapies; Enterovirus; Gene Therapy; Health and Medicine; Hoya Technosurgical Corporation; Hydroxyapatites; Immunization; Minerals; Phosphates; Phosphoric Acids; Picornaviridae; Poliovirus; RNA Viruses; Regenerative Medicine; Vaccines; Virology; Viruses EN Apatites Bioengineering Biological Products Biomedical Engineering Biomedicine Biotechnology Business Drugs and Therapies Enterovirus Gene Therapy Health and Medicine Hoya Technosurgical Corporation Hydroxyapatites Immunization Minerals Phosphates Phosphoric Acids Picornaviridae Poliovirus RNA Viruses Regenerative Medicine Vaccines Virology Viruses 427 427 1 07/24/23 20230728 NES 230728 2023 JUL 27 (NewsRx) -- By a News Reporter-Staff News Editor at Vaccine Weekly -- A patent application by the inventor KUROSAWA, Yae (Tokyo, JP), filed on June 16, 2020, was made available online on July 6, 2023, according to news reporting originating from Washington, D.C., by NewsRx correspondents. Recently, our team standardized the hydroxyapatite chromatography procedure and successfully purified dengue virus type 2 and Japanese encephalitis virus from the cell culture supernatants of virus-infected C6/36 cells and mouse brain homogenate, respectively, using one-step hydroxyapatite liquid chromatography. [Extracted from the article]