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Plaque-Forming Cells in Man I. Basic Technical Results Obtained with the Protein A Technique.
- Source :
- Scandinavian Journal of Immunology; Sep1983, Vol. 18 Issue 3, p241-248, 8p
- Publication Year :
- 1983
-
Abstract
- This paper describes the results obtained using an indirect protein A plaque-forming-cell (PFC) assay applied to human peripheral blood lymphocytes (PBL) activated with pokeweed mitogen (PWM). A maximal-response was obtained after 6 days of culture with regard to the three major classes of immunoglobulin (Ig) investigated. No difference was found between females and males. T and B lymphocytes mixed in ratios varying from 1:8 to 8:1 and unfractionated cells were investigated. A maximal PFC response of isolated lymphocytes was found in cultures of 1:4 T/B reconstituted suspensions of untreated cells, whereas the maximal response of untreated B and 2000-rad-irradialed T lymphocytes was found in cultures with a 4:1 T/B ratio. The number of plaques developed in cultures of unfractionated cells exceeded the response of 4:1 T/B reconstituted untreated lymphocytes but was far below the number of PFC developed in cultures of untreated B and 2000-rad-irradiated T lymphocytes. Normal donors developed approximately 70 × 10³ PFC/10<superscript>6</superscript> cells. The viability was 85% alter the incubation period. No difference was found in the PFC response of cultures of separated, reconstituted T/B lymphocytes set up in autologous and allogeneic combinations. [ABSTRACT FROM AUTHOR]
- Subjects :
- STAPHYLOCOCCAL protein A
MITOGENS
IMMUNOGLOBULINS
T cells
B cells
CELL culture
Subjects
Details
- Language :
- English
- ISSN :
- 03009475
- Volume :
- 18
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Scandinavian Journal of Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 16599642
- Full Text :
- https://doi.org/10.1111/j.1365-3083.1983.tb00863.x