Back to Search
Start Over
Epicutaneous immunization inducesαβ T-cell receptor CD4 CD8 double-positive non-specific suppressor T cells that inhibit contact sensitivity via transforming growth factor-β.
- Source :
- Immunology; May2005, Vol. 115 Issue 1, p42-54, 13p
- Publication Year :
- 2005
-
Abstract
- Since it was previously shown that protein antigens applied epicutaneously in mice induce allergic dermatitis mediated by production of T helper 2 (Th2) cytokines we postulated that this might induce suppression of Th1 immunity. Here we show that epicutaneous immunization of normal mice with a different protein antigen applied on the skin in the form of a patch induces a state of subsequent antigen-non-specific unresponsiveness caused by suppressor T cells (Ts) that inhibit sensitization and elicitation of effector T-cell responses. Suppression is transferablein vivobyαβ-T-cell receptor CD4<superscript>+</superscript> CD8<superscript>+</superscript> double positive lymphocytes harvested from lymphoid organs of skin patched animals and are not major histocompatibility complex-restricted nor antigen specific. Both CD25<superscript>+</superscript> and CD25<superscript>–</superscript> CD4<superscript>+</superscript> CD8<superscript>+</superscript> T cells are able to suppress adoptive transfer of Th1 effector cells mediating cutaneous contact sensitivity.In vivotreatment with monoclonal antibodies showed that the cytokines interleukin (IL)-4, IL-10 and transforming growth factor-β (TGF-β) are involved in the induction of the Ts cells. Additionally, using IL-10<superscript>–/–</superscript> mice we found that IL-10 is involved in skin induced tolerance. Furtherin vitroexperiments showed that lymph node cells of skin tolerized mice non-specifically suppress[<superscript>3</superscript>H]thymidine incorporation by antigen-stimulated immune cells and this effect can be abolished by adding anti-TGF-β, but not anti-IL-4 nor anti-IL-10 antibodies. These studies indicate the crucial role of TGF-β in skin induced tolerance due to non-antigen-specific Ts cells and also show that IL-4, IL-10 and TGF-β play an important role in the induction of epicutaneously induced Ts cell suppression. [ABSTRACT FROM AUTHOR]
- Subjects :
- ANTIGENS
GROWTH factors
CYTOKINES
IMMUNIZATION
LYMPHOCYTES
IMMUNOLOGY
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 115
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 16683503
- Full Text :
- https://doi.org/10.1111/j.1365-2567.2005.02127.x