Optimization of extraction of Selenium enriched passion fruit seed oil with supercritical CO2 extraction technology.

Selenium enriched passion fruit seed oil was prepared by supercritical CO₂ extraction method with Selenium enriched passion fruit seed as raw material. Based on single factor experiment, RSM-CCD ( response surface curve-central composite design) was used to explore the effects of temperature, pressure, static extraction time, dynamic extraction time and CO₂ flow rate on the extraction rate of Selenium enriched passion fruit seed oil. And the physical and chemical properties, fatty acid content and antioxidant activity of selenium-rich passionfruit seed oil extracted by supercritical CO₂ extraction and Soxhlet extraction were investigated. The results showed that optimal process parameters of supercritical CO₂ extraction for oil from Selenium enriched passion seed was found to be extraction temperature of 50 °C,extraction pressure of 34 MPa, static extraction of 109 min,dynamic extraction of 97 min, CO₂ flow rate of 1.0 L/min. Under this condition, the actual Selenium enriched passion seed oil was clear light yellow liquid, and the yield was 72. 33%. The content of unsaturated fatty acids extracted by supercritical CO₂ extraction was 80. 11%, which was slightly higher than that extracted by soxhlet extraction (78.71%); Acid value, peroxide value, oxidation resistance and other properties also showed that the quality of oil extracted by supercritical CO₂ extraction was better than that extracted by Soxhlet extraction. [ABSTRACT FROM AUTHOR]