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Coptis chinensis-Induced Changes in Metabolomics and Gut Microbiota in Rats.
- Source :
- American Journal of Chinese Medicine; 2023, Vol. 51 Issue 6, p1547-1576, 30p
- Publication Year :
- 2023
-
Abstract
- Rhizoma coptidis (CR) is traditionally used for treating gastrointestinal diseases. Wine-processed CR (wCR), zingiber-processed CR (zCR), and evodia-processed CR (eCR) are its major processed products. However, the related study of their specific mechanisms is very limited, and they need to be further clarified. The aim of this study is to compare the intervening mechanism of wCR/zCR/eCR on rats via faecal metabolomics and 16S rDNA gene sequencing analysis. First, faecal samples were collected from the control and CR/wCR/zCR/eCR groups. Then, a metabolomics analysis was performed using UHPLC-Q/TOF-MS to obtain the metabolic profile and significantly altered metabolites. The 16S rDNA gene sequencing analysis was carried out to analyze the composition of gut microbiota and screen out the significantly altered microbiota at the genus level. Finally, a pathway enrichment analysis of the significantly altered metabolites via the KEGG database and a functional prediction of relevant gut microbes based on PICRUSt2 software were performed in combination. Together with the correlation analysis between metabolites and gut microbiota, the potential intervening mechanism of wCR/zCR/eCR was explored. The results suggested that wCR played a good role in maintaining immune homeostasis, promoting glycolysis, and reducing cholesterol; zCR had a better effect on protecting the integrity of the intestinal mucus barrier, preventing gastric ulcers, and reducing body cholesterol; eCR was good at protecting the integrity of the intestinal mucus barrier and promoting glycolysis. This study scientifically elucidated the intervening mechanism of wCR/zCR/eCR from the perspective of faecal metabolites and gut microbiota, providing a new insight into the processing mechanism research of Chinese herbs. [ABSTRACT FROM AUTHOR]
- Subjects :
- FECAL analysis
HOMEOSTASIS
STATISTICS
MEDICINAL plants
DNA
SEQUENCE analysis
HIGH performance liquid chromatography
GUT microbiome
METABOLOMICS
ANIMAL experimentation
ONE-way analysis of variance
IMMUNE system
METABOLISM
RATS
T-test (Statistics)
PEARSON correlation (Statistics)
MASS spectrometry
DESCRIPTIVE statistics
RESEARCH funding
PEPTIC ulcer
INTESTINAL mucosa
PATH analysis (Statistics)
DATA analysis software
DATA analysis
CHINESE medicine
METABOLITES
GLYCOLYSIS
CHOLESTEROL
Subjects
Details
- Language :
- English
- ISSN :
- 0192415X
- Volume :
- 51
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- American Journal of Chinese Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 171107595
- Full Text :
- https://doi.org/10.1142/S0192415X23500702