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Positive Selection of Antigen-Specific B Lymphocytes by Means of Immunomagnetic Particles.
- Source :
- Scandinavian Journal of Immunology; Apr1988, Vol. 27 Issue 4, p439-444, 6p
- Publication Year :
- 1988
-
Abstract
- This paper presents a method for the isolation of highly enriched antigen-specific B lymphocytes from peripheral blood mononuclear cells (PBMC) by means of an immunomagnetic procedure. The method is illustrated by the isolation of B cells positive for rheumatoid factors (RF) from patients with rheumatoid arthritis and healthy blood donors. After Epstein-Barr virus transformation of isolated cells, more than 90% of the generated immunoglobulin-secreting cells produced RF in several experiments. Compared to the low frequency of B cells positive for RF in PBMC, this represents a 10³- to 10<superscript>4</superscript>-fold enrichment of antigen-specific B cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03009475
- Volume :
- 27
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Scandinavian Journal of Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 17163969