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Hyper α2,6‐Sialylation Promotes CD4+ T‐Cell Activation and Induces the Occurrence of Ulcerative Colitis.
- Source :
- Advanced Science; Sep2023, Vol. 10 Issue 26, p1-15, 15p
- Publication Year :
- 2023
-
Abstract
- α2,6‐sialylation, catalyzed by α2,6‐sialyltransferase (ST6GAL1), plays a pivotal role in immune responses. However, the role of ST6GAL1 in the pathogenesis of ulcerative colitis (UC) remains unknown. ST6GAL1 mRNA is highly expressed in UC tissues compared with the corresponding adjacent normal tissues, and α2,6‐sialylation is significantly increased in the colon tissues of patients with UC. The expression of ST6GAL1 and proinflammatory cytokines, such as interleukin (IL)‐2, IL‐6, IL‐17, and interferon‐gamma, is also increased. The number of CD4+ T cells increases in UC patients. St6gal1 gene knockout (St6gal1−/‐) rats are established by clustered regularly interspaced short palindromic repeats (CRISPR)‐associated gene knockout system. St6gal1 deficiency reduces the levels of pro‐inflammatory cytokines and alleviates colitis symptoms in UC model rats. Ablation of α2,6‐sialylation inhibits the transport of the TCR to lipid rafts and suppresses CD4+ T‐cell activation. The attenuation of TCR signaling downregulates the expression of NF‐κB in ST6GAL1‐/‐ CD4+ T‐cells. Moreover, NF‐κB could bind to the ST6GAL1 promoter to increase its transcription. Ablation of ST6GAL1 downregulates the expression of NF‐κB and reduces the production of proinflammatory cytokines to relieve UC pathogenesis, which is a potential novel target for the clinical treatment of UC. [ABSTRACT FROM AUTHOR]
- Subjects :
- ULCERATIVE colitis
LIPID rafts
GENE knockout
CD4 antigen
IMMUNE response
T cells
Subjects
Details
- Language :
- English
- ISSN :
- 21983844
- Volume :
- 10
- Issue :
- 26
- Database :
- Complementary Index
- Journal :
- Advanced Science
- Publication Type :
- Academic Journal
- Accession number :
- 172000752
- Full Text :
- https://doi.org/10.1002/advs.202302607