Thin-layer chromatographic enantioresolution of gatifloxacin using levocetirizine and levosalbutamol as chiral selectors.

In this work, we extracted, purified, and characterized two active pharmaceutical ingredients, levocetirizine and levosalbutamol (from commercial tablet formulations), and used them as chiral selectors for enantioresolution of gatifloxacin through thin-layer chromatographic (TLC) method. The silica gel was impregnated with a chiral selector for the enantioresolution of gatifloxacin. The mobile phase used was a combination of acetonitrile, methanol, and triethylamine (4:4:2, V/V, pH 9) and (2:6:2, pH 9), respectively, with levocetirizine and levosalbutamol (as chiral selector in stationary phase at pH 5). The detection limit for enantiomers of gatifloxacin was observed to be 4.2 and 4.9 μg spot^‒1, respectively, with levocetirizine and levosalbutamol as chiral selectors. This is the first report on levocetirizine and levosalbutamol as chiral selectors for TLC resolution of gatifloxacin enantiomers. [ABSTRACT FROM AUTHOR]