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Role of peroxisome proliferator?activated receptors?? in modulating P. acnes?induced pyroptosis, cell pro? liferation and apoptosis in human keratinocytes.
- Source :
- Journal of Practical Medicine / Shiyong Yixue Zazhi; 8/25/2023, Vol. 39 Issue 16, p2043-2049, 7p
- Publication Year :
- 2023
-
Abstract
- Objective To analyze the role of peroxisome proliferator-activated receptors ( PPAR- y ) expression in P. acnes-induced pyroptosis, cell proliferation and apoptosis in human keratinocytes (HaCaT) so as to provide a new theoretical basis for clinical treatment. Methods Expression patterns of PPAR- y in acne and normal skin tissues were evaluated using IHC. By lentiviral transfection, PPAR-y was upregulated in HaCaT cells, and the expression of PPAR-y was verified by Western blot. ELISA was performed to analyze the levels of P. acnes-induced pyroptotic-associated inflammatory factors IL-1p and IL-18. EdU assay and flow cytometry were used to detect the cell proliferation and apoptosis ability. Western blot was used to determine the levels of NLR family pyrin domain containing 3 (NLRP3), cysteinyl aspartate specific proteinase-1 (caspase-1), Gasdermin D (GSDMD) and PPAR-y expression. Results PPAR-y expression was significantly decreased in acne (P < 0.05). Compared with the control, the P. acnes-induced pyroptotic-associated inflammatory factors IL-ip and IL-18 were increased ( P < 0.01 ), the pyroptotic-associated proteins were increased significantly ( P < 0.05 ) . After treatment with P. acnes, the up-regulated PPAR-y reduced the expression of IL-ip and IL-18 and HaCaT cell apoptosis, promoted the cell proliferation, up-regulated the expression of NLRP3, Caspase-1 and GSDMD in human keratinocytes all in a statistically significant way (all P < 0.05). Conclusion PPAR-y can effectively regulate P. acnes-induced pyroptosis, cell proliferation and apoptosis in human keratinocytes. [ABSTRACT FROM AUTHOR]
Details
- Language :
- Chinese
- ISSN :
- 10065725
- Volume :
- 39
- Issue :
- 16
- Database :
- Complementary Index
- Journal :
- Journal of Practical Medicine / Shiyong Yixue Zazhi
- Publication Type :
- Academic Journal
- Accession number :
- 172371723