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Expression patterns and functional analysis of porcine lnc-34015.
- Source :
- Animal Biotechnology; Dec2023, Vol. 34 Issue 7, p2251-2261, 11p
- Publication Year :
- 2023
-
Abstract
- Long noncoding RNAs (lncRNAs) play important roles in immune regulation in humans and animals. The lnc-34015 was discovered to be critical for the development of muscles, based on the muscle transcriptome of pigs; however, the underlying molecular mechanism requires better understanding. Here, the sequence characteristics of lnc-34015 were analyzed and a competitive endogenous RNA regulatory network of lncRNA was predicted. The developmental expression trend and tissue expression profiles of lnc-34015 were investigated using quantitative polymerase chain reaction. The lnc-34015 sequence is overlapped with introns 11 and 12 of CWF19L1, while CWF19L1, PKD2L1, and CHUK were identified as cis-regulatory genes of lnc-34015. Bioinformatics analyses revealed that lnc-34015 binds to 15 microRNAs (miRNAs), including miR-3646, miR-377-3p, and miR-190b-3p, to regulate downstream gene expression. GO and KEGG enrichment results show that lnc-34015 was mainly involved in cell proliferation, stress response, transcriptional regulation, and alternative splicing. The expression trend of lnc-34015 in muscle was similar to that of target genes and opposite to that of miRNAs. The expression of lnc-34015 was significantly higher in the porcine small intestine and IPEC-J2 cells. Our findings suggest that lnc-34015 regulates CHUK, ZBTB20, and XIAP gene expression by competing with endogenous RNAs to regulate porcine inflammatory responses. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10495398
- Volume :
- 34
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- Animal Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 172405703
- Full Text :
- https://doi.org/10.1080/10495398.2022.2085112