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Intracellular cAMP Signaling Pathway via G s Protein-Coupled Receptor Activation in Rat Primary Cultured Trigeminal Ganglion Cells.
- Source :
- Biomedicines; Sep2023, Vol. 11 Issue 9, p2347, 14p
- Publication Year :
- 2023
-
Abstract
- G protein-coupled receptors in trigeminal ganglion (TG) neurons are often associated with sensory mechanisms, including nociception. We have previously reported the expression of P2Y<subscript>12</subscript> receptors, which are G<subscript>i</subscript> protein-coupled receptors, in TG cells. Activating P2Y<subscript>12</subscript> receptors decreased the intracellular free Ca<superscript>2+</superscript> concentration ([Ca<superscript>2+</superscript>]<subscript>i</subscript>). This indicated that intracellular adenosine 3′,5′-cyclic monophosphate (cAMP) levels can mediate Ca<superscript>2+</superscript> signaling in TG cells. Here, we report more extensive-expression patterns of G<subscript>s</subscript> protein-coupled receptors in primary cultured TG neurons isolated from 7-day-old newborn Wistar rats and further examine the roles of these receptors in cAMP signaling using the BacMam sensor in these neurons. To identify TG neurons, we also measured [Ca<superscript>2+</superscript>]<subscript>i</subscript> using fura-2 in TG cells and measured intracellular cAMP levels. TG neurons were positive for Gα<subscript>s</subscript> protein-coupled receptors, beta-2 adrenergic (β<subscript>2</subscript>), calcitonin gene-related peptide (CGRP), adenosine A<subscript>2A</subscript> (A<subscript>2A</subscript>), dopamine 1 (D1), prostaglandin I<subscript>2</subscript> (IP), and 5-hydroxytriptamine 4 (5-HT<subscript>4</subscript>) receptor. Application of forskolin (FSK), an activator of adenylyl cyclase, transiently increased intracellular cAMP levels in TG neurons. The application of a phosphodiesterase inhibitor augmented the FSK-elicited intracellular cAMP level increase. These increases were significantly suppressed by the application of SQ22536, an adenylyl cyclase inhibitor, in TG neurons. Application of agonists for β<subscript>2</subscript>, CGRP, A<subscript>2A</subscript>, D1-like, IP, and 5-HT<subscript>4</subscript> receptors increased intracellular cAMP levels. These increases were SQ22536-sensitive. These results suggested that TG neurons express β<subscript>2</subscript>, CGRP, A<subscript>2A</subscript>, D1, IP, and 5-HT<subscript>4</subscript> receptors, and the activations of these Gα<subscript>s</subscript> protein-coupled receptors increase intracellular cAMP levels by activating adenylyl cyclase. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 22279059
- Volume :
- 11
- Issue :
- 9
- Database :
- Complementary Index
- Journal :
- Biomedicines
- Publication Type :
- Academic Journal
- Accession number :
- 172412459
- Full Text :
- https://doi.org/10.3390/biomedicines11092347